Figure 2.
Platelet activation and platelet-induced IL-6 release of endothelial cells were determined. (A,B) Murine platelets were isolated 4 h after I/R and incubated with classical agonists. (A) Integrin aIIbβ3 activity and (B) P-selectin expression were measured by flow cytometry, n = 5. (C,D) Quantitative analysis of IL-6 release of the endothelia cell line MHEC5-T stimulated with either 100 ng/mL TNF-α (positive control) or 10 µM ADP, 3 µM U46619, 0.005 and 0.02 U/mL thrombin in the absence (C) or presence (D) of platelets. Statistical analysis was performed using a two-tailed Student’s t-test (A,B) and a one-way ANOVA with Dunnett’s post-hoc test (C) or a two-way ANOVA with Sidak’s post-hoc test (D), n = 5. Bar graphs depict mean values ± SEM, * p < 0.05, ** p < 0.01, and *** p < 0.001. Rest = resting, ADP = adenosine diphosphate, CRP = collagen-related peptide, Thr = thrombin, U46619 = thromboxane analog. AMI = acute myocardial infarction.