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PLD2 does not regulate TNF-α expression and release. (A) TNF-α expression in the left ventricle of Pld2+/+ and Pld2−/− mice 24 h after I/R was quantified by quantitative RT-PCR, n = 3. (B) Monocytes from whole blood of Pld2+/+ and Pld2−/− mice were isolated and stimulated with lipopolysaccharide (LPS) to analyze TNF-α release at different time points, n = 3. Bar graphs depict mean values ± SEM. Statistical analysis was performed using two-tailed Student’s t-test.
