Figure 3.

IFN-γ pretreatment improved PD-L1 t-haNK cell targeting of human breast cancer cell lines in vitro. MDA-MB-231, BT549, T47D, and MCF7 were pretreated overnight with IFN-γ prior to being incubated with PD-L1 t-haNK cells. Cell lysis was evaluated in ¹¹¹In-release assay at 25:1 E:T ratio. Values represent the %PD-L1 (MFI) for each cell treatment. Results shown are the means with SEM of triplicate measurement and representative of two independent experiments. One-way analysis of variance with Tukey’s multiple comparisons test was used for statistical analyses. *P<0.05, **P<0.01. E:T, effector to target; IFN, interferon; MFI, mean fluorescence intensity; PD-L1, programmed death-ligand 1; t-haNK, targeting high-affinity natural killer.