Skip to main content
. 2020 Apr 26;21(9):3064. doi: 10.3390/ijms21093064

Figure 1.

Figure 1

Efficient screening system in Saccharomyces cerevisiae. (A) Schematic representation of the screening reporting plasmid pRS-URA3-PB. The ura3 gene was separated by the piggyBac transposon at the TTAA site, which completely disturbed the normal expression. The piggyBac transposase with a galactose-inducible GALS promoter was inserted into the multiple cloning site. The transposon could be excised by the transposase under galactose induction, recovering the expression of the ura3 gene. (B) Schematic representation of control plasmid pRS-URA3-control. (C) The plasmid pRS-URA3-WT PBase and the plasmid pRS-URA3-control were transformed into a yeast ura− strain. After 24 h post-induction, the strains that had transferred into transposase could revert to uracil prototrophy, while the control strains could not. (D) The transposition assay in liquid media. IR, inverted repeat.