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. 2020 May 15;14:123. doi: 10.3389/fncel.2020.00123

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Copyright © 2020 Sadler, Ryals, May, Martin, Welsh, Boger, Morell, Hertzano and Cunningham.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Principal component analysis (PCA) of RiboTag IP samples. (A) PCA of whole-tissue IP samples from RiboTag Gfi1-Cre (red) and GLAST-CreER (blue) IPs. PC1 represents 54% of the total variance in the experimental data, and PC2 represents 19.87% of the total variance. Ellipses represent 95% confidence ellipses around each group of samples, and the color of each ellipse corresponds to the treatment type (heat shock in orange or control in gray). (B) Tables show the 10 genes with the highest loadings in either direction for the two PCs plotted in (A). (C) PCA of IP samples from RiboTag Gfi1-Cre whole-tissue (purple) and stroma-free (red) IPs. PC1 represents 62.71% of the total variance in the experimental data, and PC2 represents 25.81% of the total variance. Ellipses represent 95% confidence ellipses around each group of at least three samples, and the color of each ellipse corresponds to the treatment type (heat shock in orange or control in gray). (D) Tables show the 10 genes with the highest loadings in either direction for the two PCs plotted in (C).