Figure 1.

Preferential expression of GLI genes in undifferentiated hepatocellular carcinoma (HCC) cell lines. (a) Relative gene expression levels of GLI1, GLI2, and GLI3 compared to β-actin were determined in hepatoma cell lines by qRT-PCR analysis (UD; undetectable). (b) Microscopic observation of HepG2 (left), HLE (center), and HLF (right). Scale bar = 20 μm. (c) RT-PCR analysis of cell-type specific markers including E-cadherin (epithelial), Albumin, HNF4α (hepatic), Vimentin, CD44, Snail1, Twist1 (mesenchymal), and β-actin as a house keeping gene.