Figure 4.

GANT61 inhibits sphere formation by down-regulating stemness-related genes. (a) Morphological appearance of spheres formed in the presence or absence of 10 µM GANT61. HLE (upper) and HLF (lower) cells were cultured in an ultra-low-attachment culture plate for 10 days and photographed. Scale bar = 100 µm. (b) The diameter of the sphere was measured in five representative spheres of each group. (c) Quantitative PCR of stemness-related genes Oct4, Bmi1, CD44, ALDH1A1, and ALDH1A3 in HLE (left) and HLF (right) cells treated with 10 µM of GANT61. GAPDH mRNA was used as an internal control. * p < 0.05, ** p < 0.01 vs. the vehicle control (DMSO).