Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Apr 28;21(9):3126. doi: 10.3390/ijms21093126

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

GANT61 inhibits cell migration by attenuating the mesenchymal phenotype of HLE cells. (a) Transwell migration assay of HLE cells treated with 5 μM (GANT5) and 10 μM (GANT10) of GANT61. Migrated cells were stained with crystal violet. (b) The migration activity of the cells is expressed as a percentage of the cell number compared with the control. (c) Western blotting of E-cadherin, vimentin, and β-actin as an loading control. (d) Quantitarive PCR analysis of EMT-related transcription factors Snail1, Twist1, and Zeb1 in HLE (left) and HLF (right) cells treated with 10 μM GANT61. (e) Quantitative PCR analysis of Snail mRNA in HLE cells treated with control (si-Control) and Snail (si-Snail) siRNA. (f,g) Transwell migration assay of siRNA-treated HLE cells and their migration activity. * p < 0.05, ** p < 0.01 vs. vehicle control (DMSO).