Table 2. Average nucleotide identity (ANI) between pairs of the proposed type strains of Paraburkholderia sp. nov. isolates associated with D. discoideum and closely related Paraburkholderia.

These ANI scores (and percent aligned nucleotide) indicate that (a) P. agricolaris is a separate species from P. fungorum and P. insulsa, and that (b) P. hayleyella and P. bonniea are separate from each other and from P. megapolitana and P. phenoliruptrix. The conventional ANI threshold for prokaryotic species delineation is 95%.

(a) ANIb	P. agricolaris BaQS159	P. insulsa LMG28183	P. fungorum ATCC BAA-463	P. fungorum FDAARGOS245
P. agricolaris BaQS159	–	94.04 (75.05)	94.02 (75.69)	94.01 (75.65)
P. insulsa LMG28183	93.86 (67.96)	–	97.73 (83.15)	97.37(81.90)
P. fungorum ATCC BAA-463	93.99 (72.70)	97.84 (88.11)	–	97.74 (87.42)
P. fungorum FDAARGOS245	94.09 (73.21)	97.72 (87.05)	97.99 (87.55)	–