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Manhattan plots for direct calving difficulty in (A) Angus, (B) Charolais, (C) Holstein Friesian, (D) Limousin, and (E) across all breeds. The red line indicates the genome-wide significance threshold of P ≤ 5 × 10−8 and the blue is the suggestive significance threshold of P ≤ 1 × 10−6.
