Figure 2.

MDA-MB-231 cells express the three components of the arachidonate-regulated Ca²⁺-selective (ARC) channels. MCF10A, MCF7, and MDA-MB-231 cells were seeded in 6-well plates and, upon reaching the adequate cell confluence (90%), they were detached, lysed with NP-40, and denaturated by mixing with Laemmli´s buffer (LB). Subsequent Western blotting (WB) was performed using the anti-STIM1, anti-Orai1, and anti-Orai3 antibodies as described in the Materials and Methods Section. Membranes were reprobed with an anti-β-actin antibody that was used as the loading protein control. Images are representative of 4–6 independent experiments and the histogram represents the fold increase of the protein concentration found with respect to the MCF10A cells. ** p < 0.01, *** p < 0.001 using ANOVA and Tukey’s post-test, respectively.