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. 2020 May 7;21(9):3315. doi: 10.3390/ijms21093315

Figure 5.

Figure 5

Low AA concentration reduces MDA-MB-231 cell migration. MDA-MB-231 cells were transfected with siRNA A (control and AA) or siOrai3 for 48 h. A scratch was done along the culture dish at time 0 h and cells were allowed to migrate for 48 h in the presence or absence of AA (8 μM). Images of the cell cultures were captured at 0, 24, and 48 h using a bright-field microscope and a 10× objective. Bars represent 100 μm. Bar graph represents the mean ± S.E.M of the wound size expressed in μm. Images are representative of 8–12 images of each condition obtained of three independent experiments. ** p < 0.01, *** p < 0.001 with respect to the wound size found in control cells at time 0 h. $$ p < 0.01 with respect to the non-genetically modified but treated with AA. Statistical analysis was done using ANOVA and Dunnett’s post-test.