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(A) Label-free multi-photon images of collagen fibrils (green) at different z-depths of the dermis in human skin equivalents (EFT-400) that were irradiated with UVB (30 mJ/cm2), irradiated and treated with SG (20 μM) in the culture medium for 48 h, or left non-irradiated and untreated (control). Scale bars indicate 40 μm. (B) ELISA of matrix metalloproteinase (MMP)-1 secreted to the culture medium of human skin equivalents (n = 3 per group). (***; p < 0.001).
