Table 1.

X-ray data collection and refinement statistics.

Data Collection	Glucose Isomerase Embedded in WS	Lysozyme Embedded in WS	Glucose Isomerase Embedded in Alginate	Lysozyme Embedded in Alginate
Energy (eV)	12,659
Expose time	100 ms
Space group	I222	P43212	I222	P43212
Cell dimension (Å)
a	94.49	79.36	94.09	79.45
b	100.19	79.36	99.88	79.45
c	103.40	38.63	103.00	38.47
Collected images	81,798	34,484	25,000	4563
Hits images	8361	4231	10,268	3968
Indexed images	7732	3789	9946	1506
Indexed pattern	8281	4383	11,289	2066
Resolution (Å)	72.5-2.00 (2.07-2.00)	80.0-2.00 (2.07-2.00)	72.5-2.00 (2.07-2.00)	80.0-1.90 (1.97-1.90)
Unique reflections	33,248 (3247)	8808 (844)	32,970 (3233)	10,209 (989)
Completeness (%)	100.0 (100.0)	100.0 (100.0)	100.0 (100.0)	100.0 (100.0)
Redundancy	148.5 (101.5)	126.6 (88.4)	241.9 (167.8)	158.1 (112.7)
SNR	4.01 (1.59)	4.04 (1.59)	4.19 (1.28)	3.91 (1.31)
CC	0.9755 (0.5966)	0.9637 (0.6351)	0.9817 (0.4492)	0.9672 (0.4418)
CC*	0.9937 (0.8644)	0.9907 (0.8814)	0.9953 (0.7873)	0.9916 (0.7828)
Rpslit (%) a	17.22 (65.64)	17.06 (66.17)	18.99 (87.37)	17.70 (85.37)
Wilson B factor (Å2)	28.23	32.20	22.48	27.89
Refinement
Resolution (Å)	70.29-2.00	56.13-2.00	71.70-2.00	56.18-1.90
Rwork/Rworkb	17.05/21.30	18.41/23.33	19.23/22.58	18.96/23.82
R.m.s. deviations
Bond length (Å)	0.007	0.006	0.007	0.008
Bond angle (°)	0.860	0.838	0.836	0.970
B factors (Å)
Protein	29.59	38.61	26.29	33.55
Ligands	38.88	47.50	40.40	41.15
Ramachandran (%)
Preferred	95.72	95.80	96.22	95.97
Allowed	3.74	4.20	3.24	4.03
Outliers	0.53	0	0.54	0

Values for the outer shell are given in parentheses. ^a R_split = $\left(\raisebox{1ex}{$1$}\!\left/ \!\raisebox{-1ex}{$\sqrt{2}$}\right.\right)·\frac{{{\displaystyle \sum }}_{hkl}\left|I_{hkl}^{even}-I_{hkl}^{odd}\right|}{\frac{1}{2}\left|I_{hkl}^{even}-I_{hkl}^{odd}\right|}$. ^b R_work is defined as Σ||F_obs|-|F_calc||/Σ|F_obs|, where F_obs and F_calc are the observed and calculated structure-factor amplitudes, respectively; and R_free represents the R factor for the test set (10% of the data). CC refers to Pearson’s correlation coefficient, and CC* = $\sqrt{\frac{2C{C}_{1/2}}{1+C{C}_{1/2}}}$.