Figure 3.

Scratch-induced migration assay in PALM-incubated astrocyte monolayers. (A) Phase-contrast micrographs showing astrocytes exposed to untreated medium (Ctrl) and to P.N₂ (RONS), P.Air (RNS) and P.O₂ (ROS) PALM immediately after the scratch (0 h), and 6 h post-wounding. Scale bar, 100 μm. (B) Scattered plots showing the mean ± SE values of the migration rate of astrocytes incubated as indicated. A One-way Anova and Newman-Keuls Multiple Comparison Test were performed: *: p < 0.05; **: p < 0.01; ***: p < 0.001.