Figure 4.

GFAP, AQP4 and Cx43 immunoblotting and quantification in PALM-incubated astrocytes. (A) Representative Western blot analysis of GFAP, AQP4 and Cx43 expression in astroglial cultures exposed to untreated medium (Ctrl), to P.Air (RNS), to P.O₂ (ROS) and to P.N₂ (RONS). Protein samples were collected 24 h after 2 h-PALM incubation. (B) Summary of the densitometric analysis of GFAP, AQP4 and Cx43 corresponding signals normalised to the Coomassie blue-stained membrane. No significant differences by One-way Anova test were found between PALM-treated and Ctrl astrocytes.