Figure 5.

GFAP and AQP4 protein localisation in astrocyte cultures after PALM incubation. Immunostaining at 6 h after scratch for AQP4 (red) and GFAP (green) in PALM-exposed and control astrocytes. Untreated medium (Ctrl), P.N₂ (RONS), P.Air (RNS) and P.O₂ (ROS) PALM. (A) Fluorescence signal for AQP4 and GFAP in migrating cells at the wound. Nuclear staining with DAPI is also shown. (Scale bar: 100 μm). (B) Confocal images at the leading edge of wounded astrocytes as in (A). Merged images (Merge) of the two proteins’ localisation are reported (Scale bar: 25 μm). White arrows in RONS and RNS indicate enhanced foot processes at the front end of the scratch.