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Assessment of N-glycosylation. (A) SDS-PAGE analysis of the enzymatic deglycosylation of gNS using EndoH or PNGaseF deglycosylases. All samples are a mixture of native and cleaved forms. Black and white arrows refer to lane 1 (gNS) and lane 2 (PNGaseF), respectively. (B,C) Matrix-assisted laser desorption ionisation-time of flight (MALDI-TOF) MS spectra of NS (B) and gNS. Abbreviations: Nat: native; Nat*: deglycosylated Nat; Cl: cleaved; Cl*: deglycosylated Cl.
