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. 2020 Apr 28;21(9):3122. doi: 10.3390/ijms21093122

Table 3.

Parameters of AP-DNA binding by full-length and truncated forms of APE1.

Protein EC50 a, nM Maximal Extent of Binding a
APE1 0.12 ± 0.02 0.96 ± 0.03
APE1NΔ35 0.20 ± 0.03 0.77 ± 0.03
APE1NΔ61 0.21 ± 0.03 0.84 ± 0.03

a Parameters derived from electrophoretic-mobility-shift-assay (EMSA) data as described in Materials and Methods; EC50 is the effective protein concentration, at which the extent of DNA binding is half of the maximal extent. Values are the mean (± SD) of at least three independent experiments.