Table 3.
Protein | EC50 a, nM | Maximal Extent of Binding a |
---|---|---|
APE1 | 0.12 ± 0.02 | 0.96 ± 0.03 |
APE1NΔ35 | 0.20 ± 0.03 | 0.77 ± 0.03 |
APE1NΔ61 | 0.21 ± 0.03 | 0.84 ± 0.03 |
a Parameters derived from electrophoretic-mobility-shift-assay (EMSA) data as described in Materials and Methods; EC50 is the effective protein concentration, at which the extent of DNA binding is half of the maximal extent. Values are the mean (± SD) of at least three independent experiments.