Figure 3.

PLD1 deficiency decreases the population of Th1/Th17 cells and increases the population of Treg cells in collagen-induced arthritis (CIA) mice. (A) Representative flow cytometry profile of IL-17⁺ cell frequency among CD4⁺ T cells in the spleens of indicated mice with CIA (left); quantitative analysis of the CD4⁺IL-17⁺ cell population in spleen CD4⁺ T cells from mice with CIA (right). (B) Representative flow cytometry profile of CD4⁺IFN-γ⁺ T-cell frequency in the spleens of indicated mice (left); quantitative analysis of the CD4⁺IFN-γ⁺ T-cell population (right). (C) Representative flow cytometry profile of CD25⁺Foxp3⁺ cell frequency among splenic CD4⁺ T cells (left); quantitative analysis of the CD25⁺Foxp3⁺ Treg cell population in splenic CD4⁺ T cells (right). (D) Immunofluorescence staining of Foxp3 and CD25 in the spleen of the indicated mice with CIA (left); quantification CD25⁺Foxp3⁺ cell number. Treg cells are indicated by arrows. Original magnification, × 400. 3 fields per mouse spleen, 6 mice per group. * p < 0.05. The analysis was performed in CIA-challenged PLD1^+/+ and PLD1^−/− mice at day 42. Results are representative of at least six independent experiments and are shown as mean ± standard error of the mean (SEM).