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. 2020 May 2;21(9):3230. doi: 10.3390/ijms21093230

Figure 3.

Figure 3

PLD1 deficiency decreases the population of Th1/Th17 cells and increases the population of Treg cells in collagen-induced arthritis (CIA) mice. (A) Representative flow cytometry profile of IL-17+ cell frequency among CD4+ T cells in the spleens of indicated mice with CIA (left); quantitative analysis of the CD4+IL-17+ cell population in spleen CD4+ T cells from mice with CIA (right). (B) Representative flow cytometry profile of CD4+IFN-γ+ T-cell frequency in the spleens of indicated mice (left); quantitative analysis of the CD4+IFN-γ+ T-cell population (right). (C) Representative flow cytometry profile of CD25+Foxp3+ cell frequency among splenic CD4+ T cells (left); quantitative analysis of the CD25+Foxp3+ Treg cell population in splenic CD4+ T cells (right). (D) Immunofluorescence staining of Foxp3 and CD25 in the spleen of the indicated mice with CIA (left); quantification CD25+Foxp3+ cell number. Treg cells are indicated by arrows. Original magnification, × 400. 3 fields per mouse spleen, 6 mice per group. * p < 0.05. The analysis was performed in CIA-challenged PLD1+/+ and PLD1−/− mice at day 42. Results are representative of at least six independent experiments and are shown as mean ± standard error of the mean (SEM).