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. 2020 May 2;21(9):3230. doi: 10.3390/ijms21093230

Figure 4.

Figure 4

The PLD1 inhibitor ameliorates arthritis and bone erosion in collagen-induced arthritis (CIA) mice. (A–C) Effect of the PLD1 inhibitor on the incidence, arthritic score, and the number of affected limbs of CIA mice. DBA/1J mice were immunized on day 0 and boosted on day 21 with type II collagen; intraperitoneal injection of the vehicle or PLD1 inhibitor (5 mg/kg) was done every other day start on day 21 post immunization. The clinical symptoms of the disease including incidence (A), arthritic score (B), and the number of affected limbs (C) were scored. (D) Representative photographs showing the gross features of the hind paws at day 45. (E) Representative micro-computerized tomography (CT) images of the femurs and knee joints of mice at day 45. (F) Effect of the PLD1 inhibitor on the micro-CT parameters of trabecular bone mass in CIA mice at day 45. (G) Hematoxylin and eosin staining of knee joints from the indicated mice at day 45. Boxed areas are shown at approximately two times higher magnification. (H) Histological assessment of knee joints. n = 15 per group. * p < 0.05. Quantitative results are shown as mean ± standard error of the mean (SEM).