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. 2020 May 21;13:4509–4521. doi: 10.2147/OTT.S240261

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© 2020 Lin et al.

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CircGprc5a directly targeted miR-1283 in HCC. (A) Putative targeting sites for circGprc5a and miR-1283. (B) Analysis of luciferase activity in HCC cells co-transfected with circGprc5a-WT or circGprc5a-Mut vector. (C) Immunoprecipitation (RIP) assay for the degree of enrichment of circGprc5a and miR-1283 RNA. Anti-immunoglobulin G (IgG) was used as a control.**P <0.01. (D) Expression level of miR-1283 in HCC cells knocked down with circGprc5a. (E) Expression levels of miR-1283 in HCC tissues and adjacent normal tissues (n = 64). (F) Pearson correlation analysis of circGprc5a and miR-1283 in liver cancer tissues (n = 64) (r = −0.660, P <0.01). **P <0.01.