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. 2020 May 11;21(9):3400. doi: 10.3390/ijms21093400

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Lactate dehydrogenase (LDH) assay and the Western blot of kidney injury molecule (KIM)-1 and N-cadherin in rat mesangial cells (RMCs). Cells were treated with bicalutamide (Bic) at concentrations of 30 and 60 μM at 24 h. (a) LDH release was increased by Bic (n = 3, * p < 0.05). (b) A representative blot of protein expressions of KIM-1 and N-cadherin. GAPDH was used as an internal control. (c) Quantitative data of Western blotting of KIM-1(n = 3, * p < 0.05). (d) Quantitative data of Western blotting of N-cadherin (n = 3, * p < 0.05). When RMCs were treated with Bic, N-cadherin dose-dependently decreased, however KIM-1 was significantly induced in the group treated with 60 μM.