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. 2020 May 11;21(9):3400. doi: 10.3390/ijms21093400

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Annexin V analysis of apoptosis of rat mesangial cells (RMCs) mediated by bicalutamide (Bic) and FG4592. Cells were co-treated with Bic (60 μM) for 24 h in the presence and absence of FG4592 (2.5 μM). (a) Upper panel: early apoptosis. (b) Middle panel: late apoptosis/dead. (c) Lower panel: total apoptosis. A statistical analysis of the cytometric data showed that FG4592 partially reversed apoptotic cells induced by Bic. Triplicate data were statistically analyzed and results are expressed as the mean ± standard deviation. ** p < 0.01 compared to the control. ^# p < 0.05 compared to 60 μM Bic-treated cells.