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. 2020 Apr 27;21(9):3081. doi: 10.3390/ijms21093081

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The colony-forming unit-fibroblast (CFU-F) assay of ASCs obtained by Rigenera^® ((a) thigh and (b) abdomen) and the enzymatic method ((d) thigh and (e) abdomen). CFU-F values were determined at day 15 after plating using the Toluidine Blue staining method. ASCs extracted from the thigh (a–d) formed larger colonies containing more cells than those extracted from the abdomen (b–e); (c) CFU-F numbers at day 15 obtained by the Rigenera^® device; CFU-F numbers showed significant differences between the two groups (thigh and abdomen). (f) CFU-F numbers at day 15 obtained by the enzymatic method; CFU-F numbers showed no significant differences between the two groups (thigh and abdomen). Data are presented as the mean ± SE, with n = 6 (** p < 0.01).