Table 2.
CAZyme and symbiosis marker genes considered in RT-qPCR experiments.
| Gene Name | Putative Function | Transcript ID † | MYC Normalized Reads ‡ | FLMNormalized Reads ‡ |
|---|---|---|---|---|
| Tulasnella calospora | ||||
| TcGH6 | cellobiohydrolase | 69053 | 2.71 | 12.98 |
| TcGH10a | endo-1,4-β-xylanase | 14789 | 3.37 | 24.59 |
| TcGH11 | endo-1,4-β-xylanase | 80414 | 5.43 | 0.62 |
| TcGH45* | endoglucanase | 224031 | 26.58 | 6.49 |
| TcAA9a | Lytic polysaccharide monooxygenase (LPMO) | 75481 | 3.45 | 12.18 |
| TcAA9b | LPMO | 6298 | 102.01 | 61.82 |
| TcAA9f | LPMO | 4643 | 16.35 | 0.33 |
| TcAMT1 | ammonium transporter | 241330 | 584.63 | 324.12 |
| TcAMT2 | ammonium transporter | 183841 | 525.27 | 184.32 |
| TcAAT2 | amino acid transporter | 81605 | 363.85 | 77.20 |
| Serapias vomeracea | ||||
| SvNod1 | early nodulin 55-2, putative | DN89686_c0_g1_i1 | - | - |
| SvEXO | exocyst subunit exo70 | DN73752_c2_g2_i1 | 42.61 | 7.38 |
* Oligonucleotides worked only in the Cattleya-Tulasnella model. † T. calospora transcripts IDs refers to the AL13/D transcriptome deposited in JGI portal (Kohler et al. 2015) while S. vomeracea IDs refers to the assembled transcriptome [26] available at NCBI (accession GSE87120). ‡ MYC reads and FLM reads refer to the T. calospora RNA-seq transcriptome analysis [17,26]. MYC reads and FLM reads columns show the mean number of reads observed in symbiotic and free-living conditions, respectively.