Figure 1.

Representative results of LGIR with peripheral blood mononuclear cells (PBMC) from a monkey. PBMCs (2 × 10⁶ cells/well) from a healthy monkey HM-1 were cultured with iPSC-RPE cells for 5 days. Before the assay, iPSC-RPE cells were irradiated (20 Gy) and 1 × 10⁴ cells were used for a 24-well culture plate. After 5 days of coculture, PBMC were harvested and stained with anti-CD4 (helper T cell-marker), anti-CD8 (cytotoxic T cell-marker), anti-CD11b (monocyte-, macrophage-, NK cell-, and granulocyte-marker), anti-CD20 (B cell-marker), anti-NKG2A (natural killer (NK) group 2 member A; NK cell-marker), and anti-Ki-67 (proliferation marker) antibodies. As a positive control (PC), irradiated allogenic B cells were used. The samples were analyzed by a fluorescence-activated cell sorting (FACS) flow cytometer. Numbers (%) in the scatterplots indicate double-positive cells (e.g., CD4/Ki-67).