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. 2020 May 9;21(9):3365. doi: 10.3390/ijms21093365

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Experimental schedule to examine the effect of BME (50 mg/kg) on spatial working memory in adolescent (5-week-old) or adult (8-week-old) mice using the modified Y maze. (A): Adolescent mice were orally administered BME for 28 days. The spatial cognitive performance of the animals was elucidated at days 7 and 28 after the first BME treatment. (B) and (C): Adolescent (B) or adult mice (C) mice were orally administered BME for 7 days, respectively, and then a modified Y maze test was conducted at day 7 after the first BME treatment. (D): Adolescent mice were orally administered BME for 7 days, and a modified Y maze test was conducted at day 28 after the first BME treatment.