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. 2020 Apr 28;21(9):3121. doi: 10.3390/ijms21093121

Figure 3.

Figure 3

Thermotolerance of WT and transgenic Arabidopsis plants. (A) Schematic representation of the construct used for Arabidopsis transformation. LB left border, pMAS mannopine synthase promoter, Tnos nopaline synthase gene (NOS) terminator, Hyg Hygromycin-resistance gene, RB right border. (B) Relative expression levels of TaHsfA6f in the leaves of T3 transgenic Arabidopsis lines via qPCR analysis. Each value is the mean of triplicate experiments. Bars indicate standard deviations. WT wild-type, N.D = Not Detected. (C) Comparison of thermotolerance among WT and three transgenic lines (L1, L2, and L3). (D) Survival rates of WT and three transgenic lines after heat stress at 40/32 °C (day/night) for 10 days. For each experiment, 20 plants per line were used; values are means ± SD from three independent measurements (** p < 0.01 by Student’s t-test).