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. Author manuscript; available in PMC: 2020 Jul 3.
Published in final edited form as: Sci Immunol. 2020 Jan 3;5(43):eaax5430. doi: 10.1126/sciimmunol.aax5430

Figure 2. Chemical analysis of antigenic substances in balsam of Peru.

Figure 2.

a. Normal phase silica TLC plate resolves balsam of Peru oil (BPO), crude balsam of Peru (BP), synthetic benzyl cinnamate (BC) and synthetic benzyl benzoate (BB). b. Structures of benzyl cinnamate and benzyl benzoate are shown with the expected mass of sodium adducts [M+Na]+, which were detected in positive-mode nanoelectrospray ionization mass spectrometry. c. T cell clones that are autoreactive to CD1a (BC2) or foreign antigen (CD8–2) were tested for response to antigens (μg/ml) or sphingomyelin (sphingomy) by IFN-γ ELISA in cellular (e) or CD1a-coated plate (c, d) assays. Data are representative of three or more experiments, each shown as the mean of triplicate samples +/− standard deviation. The significance of lipid concentration on IFN-γ release was tested by one-way ANOVA (panel c). The significance of benzyl cinnamate and benzyl benzoate concentration on IFN-γ release and of the effects of CD1b or CD8–2 T-cells were tested by two-way ANOVA (panels d,e).