Table 5.
Quantification of the exposure of β-1,3-glucan and chitin of Candida species, with and without caspofungin treatmenta.
| Candida species | Glucan exposure (mean fluorescence intensity) | Chitin exposure (mean fluorescence intensity) | ||
|---|---|---|---|---|
| No treatment | Caspofungin treatment | No treatment | Caspofungin treatment | |
| C. albicans | 179 ± 20 | 450 ± 34# | 167 ± 17 | 488 ± 51# |
| C. dubliniensis | 211 ± 14 | 374 ± 52# | 160 ± 26 | 404 ± 40# |
| C. glabrata | 273 ± 21* | 257 ± 39 | 89 ± 18* | 79 ± 20 |
| C. tropicalis | 185 ± 41 | 607 ± 66# | 178 ± 22 | 516 ± 54# |
| C. lusitaniae | 171 ± 29 | 468 ± 49# | 159 ± 31 | 438 ± 35# |
| C. parapsilosis | 201 ± 13 | 189 ± 30 | 172 ± 28 | 202 ± 45 |
| C. guilliermondii | 263 ± 36* | 537 ± 72# | 160 ± 29 | 445 ± 49# |
a
The average relative β-1,3-glucan and chitin contents of individual cells from different Candida species were determined by measuring the intensity of Fc:Dectin1–Alexa488 and WGA-Texas Red fluorescence. Measurements were made on untreated control cultures and after growth with caspofungin at the specific IC50 for each species (as determined in Table 1). Statistical differences are shown for comparison to untreated cells of C. albicans (*P = 0.05) or untreated cells of the same species (#P = 0.05), and data are means with standard deviations (n = 50).