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Purification of the extracellular protease produced by Psychrobacter sp. P94-6PB.
| Purification step | Fraction volume (ml) | Protein concentration (mg/ml) | Inserted protein amount in activity assay (mg) | Total activity (U) | Specific activity (U/mg) | Purification fold | Yield (%) |
| Crude extract | 80 | 70 | 3.5 | 191 | 54.6 | 1 | 100 |
| Ni-NTA | 37.5 | 2 | 0.1 | 56 | 560.0 | 10 | 29 |
One unit of activity was defined as an increase of 0.01 in absorbance units at 440 nm in the azocasein assay.
