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. 2020 May 12;11:850. doi: 10.3389/fimmu.2020.00850

Figure 4.

Figure 4

SIV-specific CD8+ T cells response in semen of SIVmac251-infected macaques. Intracellular staining for Th1 cytokines in semen CD8+ T cells in response to a 5-h stimulation with SIV gag peptide or without stimulation (unstimulated). (A,D) IFN-γ and MIP-ß expression in seminal plasma (A) and blood (D) CD8+ T cells of all studied animals (n = 7), with (lower panels) or without (upper panels) stimulation with gag. (B,E) Polyfunctionality of semen (B) and blood (E) CD8+ T cells in seven chronically infected macaques. IL-2 was not included because it was not produced in sufficient amounts. Each color represents one animal and the mean is shown. Shown are results after subtraction of results for the non-stimulated cultures. (C,F) Pie charts showing the polyfunctionality of semen CD8+ T cells in seminal plasma (C) and blood (F) of chronically-infected macaques with SIV-specific CD8 T cells (#30690, #31044, and #BC461) at steady state (128 dpi, upper panel) and the late chronic stage (413 dpi, lower panel). Macaques were infected with 5,000 AID50 IV.