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CRISPR-Cas9 schematics and editing efficiency. (A) CRISPR-Cas9 genome editing working model modified from Tong et al. (2018) showcasing the two major routes genome editing via DNA double-strand break repair utilizing non-homologous end joining (NHEJ) or homology-directed repair (HDR) (Tong et al., 2018). (B) Single-base editing utilizing modified Cas9 fusion proteins (Komor et al., 2016; Gaudelli et al., 2018).
