Figure 3. Hit compounds identified by SAFIRE-CFU.

A) Depending on the library screened, hits may include antibiotics or chemicals with antibiotic activity. B) Most hit compounds, like vehicle, do not prevent bacterial growth in standard microbiological media. C) Some of these hits act on bacteria in the host setting. D) Other hits stimulate the host cell to kill bacteria. Pro-drugs activated by the tissue culture medium, host cell, and/or bacteria could also be identified.