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UV-vis spectra of an aliquot of (HbII-HbIII)-O2 transferred into a 2.0 M solution of sodium formate at pH 4.6. Spectra were normalized. This solution is 60% less concentrated than the crystallization condition to avoid precipitation of the sample and observe the effect of the crystallization solution on the oxidation state of L. pectinata (HbII-HbIII)-O2 at acidic pH.
