Table 1. Primer sequences used in PCR analysis.
| Target genes | Forward Primer | Revers Primer | Product size | Accession no. |
|---|---|---|---|---|
| TLR2-1 | ACATGTGTGAATGGCCTGAA | TTGAGAAATGGCAGTTGCAG | 151 | NM_204278.1 |
| TLR4 | AGTCTGAAATTGCTGAGCTCAAAT | GCGACGTTAAGCCATGGAAG | 190 | NM_001030693.1 |
| TLR21 | TGCCCCTCCCACTGCTGTCCACT | AAAGGTGCCTTGACATCCT | 113 | NM_001030558.1 |
| IL-1β | GTGAGGCTCAACATTGCGCTGTA | TGTCCAGGCGGTAGAAGATGAAG | 214 | NM_204524.1 |
| CXCLi2 | CTGTCCTGGCCCTCCTCCTGGTT | TGGCGTCAGCTTCACATCTTG | 146 | NM_205498.1 |
| TNFSF15 | CCTGAGTTATTCCAGCAACGCA | ATCCACCAGCTTGATGTCACTAAC | 292 | NM_001024578.1 |
| TGFβ-2 | AGGAATGTGCAGGATAATT | ATTTTGGGTGTTTTGCCAA | 269 | NM_001031045.3 |
| TGFβ-3 | CAGATCCTGGCGCTCTACA | GAGGCCCTGGATCATGTCA | 141 | NM_205454.1 |
| TGFβ-4 | ATGAGTATTGGGCCAAAG | ACGTTGAACACGAAGAAG | 109 | NM_001318456.1 |
| AvBD2 | GTTCTGTAAAGGAGGGTCCTGCCAC | ACTCTACAACACAAAACATATTGC | 238 | NM_204992.2 |
| AvBD7 | ACCTGCTGCTGTCTGTCCTC | TGCACAGCAAGAGCCTATTC | 173 | NM_001001194.1 |
| Claudin1 | GACTCGCTGCTTAAGCTGGA | AAATCTGGTGTTAACGGGTG | 276 | NM_001013611.2 |
| Claudin5 | GTCCCGCTCTGCTGGTTC | CCCTATCTCCCGCTTCTGG | 84 | NM_204201.1 |
| ZO2 | GAAGCAGAGGTCGTAGTAGG | CTGTCCATAGCCACCATCC | 140 | NM_204918.1 |
| JAM2 | AGCCTCAAATGGGATTGGATT | CATCAACTTGCATTCGCTTCA | 59 | NM_001006257.1 |
| RPS17 | AAGCTGCAGGAGGAGGAGAGG | GGTTGGACAGGCTGCCGAAGT | 136 | NM_204217.1 |