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. 2020 May 25;10:8637. doi: 10.1038/s41598-020-65596-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Agglomeration and SEM images of the PS NPs studied. Agglomeration was studied over time (0, 1, 3 and 24 h) in different aqueous suspensions; ultrapure water (panel A), artificial marine water (panel 2) and serum hemolymph, 1:1 serum of hemolymph:anti-aggregation solution (panel C) by Static Light Scattering (SLS), also SEM images of the nanoparticles in the different cultures media were taken after 24 h.