Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 May 25;11:2607. doi: 10.1038/s41467-020-16124-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — a In digital PLA, each detected protein molecule is bound by a pair of DNA oligonucleotide-conjugated antibodies (i.e., proximity probes), the oligonucleotides are ligated with the help of a connector, and the ligated products are quantified by droplet digital-PCR (ddPCR). b The specificity of dPLA was tested by using IL-6 and TNF-α antibodies against IL-6, TNF-α and IL-10 spike-in standards. Only IL-6 spike-in produced a high signal with IL-6 antibody, and only TNF-α produced a high signal with TNF-α antibody. c, d Calibration curves for IL-6 (c) and TNF-α (d) spiked in with healthy human plasma. The solid lines in (c, d) are the linear regression of the calibration curves. Data are presented as mean ± s.e.m., and each concentration has three technical replicates.