Table 2.
Sequences of primers used for PCR amplification and sequencing gene fragments in this study. Those marked with ‘*’ were specifically modified for Hirudinaria species (F = Forward, R = Reverse).
| Genes | Amplicon length (bp) | Primer name | Primer sequence (5’ to 3’) | Reference |
|---|---|---|---|---|
| COI | ||||
| 1 | 720 | LCO1490hiru (F) | ATT CTA CTA GTC ATA AAG ATA TTG G | This study |
| HCO2198hiru (R) | AAA ATC AAA ATA TAT ACT TCT GGA TG | This study | ||
| 2 | 805 | LCOinHiru (F) | GAA ATG AGC GAG TCC TTT ATT TG | This study |
| HCO-out* (F) | TCT GGA TAG TCT GAA TAT CG | Nakano (2012) | ||
| 28S | ||||
| 1 | 802 | 28F2-2 (F) | GCA GAA CTG GCG CTG AGG GAT GAA C | Passamaneck et al. (2004) |
| 28R2 (R) | GAG GCT GTK CAC CTT GGA GAC CTG CTG CG | Passamaneck et al. (2004) | ||
| 2 | 824 | 28F2-3 (F) | ATC GAA AGG GAA TCG GGT TAA TAT TCC | Jördens et al. (2004) |
| 28R3 (R) | GAT GAC GAG GCA TTT GGC TAC C | Passamaneck et al. (2004) | ||