Figure 7.

Adoptive transfer of enriched CD4⁺ and/or CD8⁺ splenic T cells to DO11.10 mice restores the local skin memory response. (A) Protocol. (B) CD4⁺ or CD8⁺ T cells were enriched from naïve BALB/c splenocytes using magnetic cell depletion. The enrichment efficiency of the transferred cells on day−8 was assessed by flow cytometry. (C) Ear swelling upon ear sensitization + the 1^st challenge and re-challenge. The numbers of transferred cells per mouse were indicated in each figure. CD4⁺ + CD8⁺ T cell-injected [1^st challenge (n = 5), re-challenge (n = 4)], other groups (n = 6 each). Data represent means ± SE. *P < 0.05 (two-tailed paired t-test).