Table 4.

Genotypic and phenotypic characterization of S. pneumoniae strains used for confirmation of gene markers.

Strain	PCR analysis						Optochin test		WGS analysis
	Pneumo	Pneumo	Pneumo	Pneumo	Pneumo	Pneumo	Aerobic	CO2	“Xisco” gene	ANIb (%)
	127	1011	1012	1013	1014	1961
CCUG 28588T	+	+	+	+	+	+	S	S	+	99.9
CCUG 1350	+	+	+	+	+	+	S	S	+	98.3
CCUG 6798	+	+	+	+	+	+	S	S	+	98.4
CCUG 7206	+	+	+	+	+	+	S	S	+	98.3
CCUG 11780	+	+	+	+	+	+	S	S	+	98.2
CCUG 32672	+	+	+	+	+	+	S	S	+	98.4
CCUG 33774	+	+	+	+	+	+	S	S	+	98.4
CCUG 35180	+	+	+	+	+	+	S	S	+	98.4
CCUG 35229	+	+	+	+	+	+	S	S	+	98.5
CCUG 35272	+	+	+	+	+	+	S	S	+	98.2
CCUG 35561	+	+	+	+	+	+	S	S	+	98.5
CCUG 36618	+	+	+	+	+	+	S	S	+	98.4
CCUG 36800	+	+	+	+	+	+	S	S	+	98.4
CCUG 45673	+	+	+	+	+	+	S	S	+	98.3
CCUG 63093	+	+	+	+	+	+	S	S	+	98.3
CCUG 63665	+	+	+	+	+	+	S	S	+	98.4
CCUG 68718	+	+	+	+	+	+	S	S	+	98.2
CCUG 69380	+	+	+	+	+	+	S	S	+	98.4
CCUG 69381	+	+	+	+	+	+	S	S	+	98.3
CCUG 69382	+	+	+	+	+	+	S	S	+	98.3

ANIb values calculated with JSpeciesWS against the type strain of S. pneumoniae NCTC 7465^T are also indicated.