Table 1.
Determination of the 50% neutralizing antibody titer in mouse sera by the microculture neutralization test.
| Serum collection time | rAd3H immunization | HAdV-3 wildtype GZ01 inoculation | ||
|---|---|---|---|---|
| Intranasal | Intramuscular | Intranasal | Intramuscular | |
| D21 | 1:250 | 1:270 | 1:1000 | 1:1428 |
| D28 | 1:200 | 1:250 | 1:1111 | 1:1000 |
| D35 | 1:167 | 1:333 | 1:1111 | 1:1667 |
| D42 | 1:158 | 1:248 | 1:916 | 1:1024 |
The mice were either immunized with the rAd3H recombinant vaccine or inoculated with HAdV-3 wildtype strain GZ01 intranasally or intramuscularly. At day 21, 28, 35, and 42 after the prime inoculation/immunization, the neutralizing antibodies against HAdV-3 were titrated.