Table 4.
Kinetic parameters for the uptake and binding of radioligands in adherent transporter-transfected HEK 293 cells.
| Km ± SD (µM) | Vmax ± SD (pmol/min/106 cells) | Kd ± SD (µM) | Bmax ± SD (pmol/min/106 cells) | |
|---|---|---|---|---|
| Cell line 1 | ||||
| hSERT | 8.1 ± 3.0 | 483 ± 57 | 0.12 ± 0.03 | 112 ± 31 |
| hDAT | 4.7 ± 3.3 | 323 ± 98 | 0.1 ± 0.08 | 0.5 ± 0.1 |
| hNET | 4.6 ± 1.2 | 205 ± 17 | 0.05 ± 0.02 | 0.4 ± 0.1 |
| Cell line 2 | ||||
| hSERT | 4.1 ± 1.0 | 160 ± 16 | 1.2 ± 0.4 | 3.6 ± 1.9 |
| hDAT | 5.5 ± 0.8 | 370 ± 68 | 0.051 ± 0.007 | 0.7 ± 0.2 |
| hNET | 1.4 ± 0.7 | 104 ± 9 | 0.07 ± 0.02 | 0.4 ± 0.1 |
| Cell line 3 | ||||
| rSERT | 3.4 ± 1.5 | 179 ± 59 | 1.13 ± 0.07 | 3.0 ± 0.9 |
| rDAT | 8.8 ± 3.9 | 604 ± 23 | 0.07 ± 0.02 | 1.0 ± 0.2 |
| rNET | 0.3 ± 0.1 | 18.1 ± 4.7 | 0.13 ± 0.07 | 0.4 ± 0.1 |
Radioligand uptake and binding assays were conducted as described in Radioligand Uptake and Binding in Adherent Transporter-Transfected HEK Cells. Values represent means ± SD of at least three independent experiments.