Figure 2.

U. parvifolia inhibits [Ca²⁺]_i mobilization and reduces ATP release and TxB2 production. (A) Fura 2/AM-loaded rat platelets pre-treated with vehicle or various U. parvifolia extract concentrations and stimulated with collagen (2.5 µg/ml) for 3 min. Assessment of ATP concentration (B), and thromboxane-B2 production (C) was done in supernatant of stimulated washed platelets suspension pre-treated with vehicle or various U. parvifolia extract concentrations and stimulated with collagen for 5 min on a luminometer or TxB2 ELISA kit, respectively. Results are represented as mean ± SD values from at least four independent experiments. **p < 0.01 and ***p < 0.001 versus control.