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. 2020 May 19;8:318. doi: 10.3389/fcell.2020.00318

FIGURE 1.

FIGURE 1

Conditioned medium inhibits DR5-mediated apoptosis in Colo205 cells. Colo205 (A, upper) and BJAB cells (A, below) were treated with TRAIL DHER variant for 24 h in the presence of fresh medium (FM) or conditioned medium derived from either Colo205 (CMc), BJAB expressing both DR5 isotypes (CMb DR5), or BJAB cells deficient for DR5 short isoform (CMb DR5s DEF) cells. Conditioned medium was collected after cultivation of cells at a density of 150,000 cells/mL for 48 h. Cell death was measured by MTS assay. Data expressed as the mean ± SD of triplicate samples. Similar results were obtained in three independent experiments. (B) Total cell extract and conditioned medium (CM) of three colon carcinoma cell lines (upper) and Burkitt lymphoma cell lines (below) were analyzed for DR4, DR5, and H2A expression with Western blot. The absence of H2A in CM indicates no contamination of cellular nucleosome proteins. Similar results were obtained in three independent experiments.