Table 1.
List of the analytes included in Mix I and Mix II, with the relative CAS number and the internal standard used for their quantitation. The concentrations at the different calibration levels are also reported.
| Target analyte | CAS number | Internal standard | ||||
|---|---|---|---|---|---|---|
| Mix I | 5β-androstan-13,17-dione | 1229–12–5 | Testosterone-D3 | |||
| 5α-androstane-3α,17β-diol (5α-adiol) | 1852–53–5 | Testosterone-D3 | ||||
| 5β-androstane-3α,17β-diol (5β-adiol) | 1851–23–6 | Testosterone-D3 | ||||
| dehydroepiandrosterone (DHEA) | 53–43–0 | Testosterone-D3 | ||||
| 5-androsten-3,17-diol | 512–17–5 | Testosterone-D3 | ||||
| epitestosterone (E) | 481–30–1 | Testosterone-D3 | ||||
| 4,6-androstadien-3,17‑dione (6-D) | 633–34–1 | Testosterone-D3 | ||||
| dihydrotestosterone (DHT) | 521–18–6 | Testosterone-D3 | ||||
| 4-androsten-3,17-dione | 63–05–8 | Testosterone-D3 | ||||
| Δ6-testosterone | 2484–30–2 | Testosterone-D3 | ||||
| testosterone (T) | 58–22–0 | Testosterone-D3 | ||||
| 7α-hydroxytestosterone | 62–83–9 | Testosterone-D3 | ||||
| 7β‑hydroxy-dehydroepiandrosterone (7β-OH-DHEA) | 2487–48–1 | Testosterone-D3 | ||||
| formestane | 566–48–3 | Testosterone-D3 | ||||
| 4-hydroxytestosterone | 2141–17–5 | Testosterone-D3 | ||||
| 16α-hydroxyandrosten-3,17-dione | 63–02–5 | Testosterone-D3 | ||||
| Mix II | androsterone (Andro) | 53–41–8 | 17α-methyl-testosterone | |||
| etiocholanolone (Etio) | 53–42–9 | 17α-methyl-testosterone | ||||
| Calibration level | 1 | 2 | 3 | 4 | 5 | 6 |
| Mix I(ng/mL) | 2 | 5 | 10 | 25 | 50 | 125 |
| Mix II(ng/mL) | 100 | 200 | 500 | 1000 | 1500 | 2250 |