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. 2020 May 19;11:465. doi: 10.3389/fphys.2020.00465

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Copyright © 2020 Mendonca Costa, Anderson, Meijborg, O’Shea, Shattock, Kirchhof, Coronel, Niederer, Pavlovic, Dhanjal and Winter.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of low-flow ischemia on electrogram morphology in isolated mouse hearts. Changes in electrogram morphology during low-flow global ischemia in perfused mouse hearts. (A) Representative bipolar electrogram recordings showing the change in electrogram morphology during a 120-s period of low-flow ischemia and subsequent tissue reperfusion in a single mouse heart. The red shading indicates the calculated electrogram area. (B–D) Mean data from seven mouse hearts showing the changes in bipolar electrogram amplitude (B), absolute electrogram area (EA) (C), and amplitude-normalized EA (norm_EA) (D) in response to ischemia-reperfusion. Different from 0; *p < 0.05, **p < 0.01, ***p < 0.001. Data are mean ± SEM. Actual replicates are shown in gray. n = 7 hearts.