Table 3.

Primers used in qRT-PCR for quantification of TLRs and cytokines mRNA expression.

Primers	Oligonucleotides (5′−3′)	Annealing temperature
β-actin	Forward: ACCGGACTATTACCAACACC Reverse: GACTGCTGCTGACACCTTCA	56°C
TLR 2	Forward: GCTCAACAGCTTCTCCAAGG Reverse: CCACCAGGATGAGGATGAAC	57°C
TLR 4	Forward: GGATGGACCGCAGCATGTTC Reverse: CAACCTGAGCAGCCTGTACG	56°C
IL-4	Forward: GGAGAGCATCCGGATAGTGA Reverse: TGACGCATGTTGAGGAAGAG	54°C
IL-10	Forward: CATGCTGCTGGGCCTGAA Reverse: CGTCTCCTTGATCTGCTTGATG	57°C