Figure 3.

Histological and Immunohistochemical Analysis of the Vestibular Nucleus Sacrificed at 100 Days

(A) Immunohistochemical analysis using an anti-NDUFS4 antibody. Scale bars, 50 μm. (B) Analysis of astrogliosis by immunodecoration with an anti-GFAP antibody. Scale bars, 10 μm. (C) Analysis of microgliosis by immunodecoration with an anti-CD68 antibody. Scale bars, 10 μm. (D) H&E staining. Note the presence of spongiform vacuolization in VN of untreated Ndufs4^−/− animals, which is absent in AAV-PHP.B-hNDUFS4-treated animals. Scale bars, 50 μm. (E) PathoGreen staining. No positive cells are detected in the VN of either treated or untreated mice at this stage. Scale bars, 20 μm.